ENZYME ASSAYS USING PERMEABILIZED CELLS OF NEUROSPORA

被引：37

作者：

BASABE, JR ^{[1
]}

LEE, CA ^{[1
]}

WEISS, RL ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,DEPT CHEM,LOS ANGELES,CA 90024

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90670-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is described for the preparation of homogeneous, steady-state cultures of germinated conidia of Neurospora crassa. Permeabilization of such cells has been accomplished by a combination of toluene-ethanol and freeze-thaw treatments. These permeabilized cells have been used for the determination of enzyme activities. The method has been shown to be rapid and rellable and is applicable to nuclear, mitochondrial, and cytosolic enzymes. © 1979.

引用

页码：356 / 360

页数：5

共 17 条

[1] METHOD FOR DECRYPTIFICATION OF ALPHA-GLUCOSIDASE IN YEAST WITH DIMETHYL SULFOXIDE
ADAMS, BG
[J]. ANALYTICAL BIOCHEMISTRY, 1972, 45 (01) : 137 - &
[2] GROWTH-REGULATION IN NEUROSPORA-CRASSA EFFECTS OF NUTRIENTS AND OF TEMPERATURE
ALBERGHINA, FA
[J]. ARCHIV FUR MIKROBIOLOGIE, 1973, 89 (02): : 83 - 94
[3] Archibald RM, 1944, J BIOL CHEM, V156, P121
[4] CARBAMOYL PHOSPHATE COMPARTMENTATION IN NEUROSPORA - HISTOCHEMICAL LOCALIZATION OF ASPARTATE AND ORNITHINE TRANSCARBAMOYLASES
BERNHARDT, SA
DAVIS, RH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (07) : 1868 - +
[5] CHINARD FP, 1952, J BIOL CHEM, V199, P91
[6] MUTANTS OF NEUROSPORA CRASSA DEFICIENT IN ORNITHINE-DELTA-TRANSAMINASE
DAVIS, RH
MORA, J
[J]. JOURNAL OF BACTERIOLOGY, 1968, 96 (02) : 383 - &
[7] ARGINASELESS NEUROSPORA - GENETICS, PHYSIOLOGY, AND POLYAMINE SYNTHESIS
DAVIS, RH
LAWLESS, MB
PORT, LA
[J]. JOURNAL OF BACTERIOLOGY, 1970, 102 (02) : 299 - &
[8] CARBAMYL PHOSPHATE SYNTHESIS IN NEUROSPORA CRASSA .2. GENETICS METABOLIC POSITION AND REGULATION OF ARGININE-SPECIFIC CARBAMYL PHOSPHOKINASE
DAVIS, RH
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 107 (01) : 54 - &
[9] A MUTANT FORM OF ORNITHINE TRANSCARBAMYLASE FOUND IN A STRAIN OF NEUROSPORA CARRYING A PYRIMIDINE-PROLINE SUPPRESSOR GENE
DAVIS, RH
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1962, 97 (01) : 185 - &
[10] Davis RH., 1970, METHODS ENZYMOLOGY A, V17, P79, DOI DOI 10.1016/0076-6879(71)17168-6

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