INHIBITION OF PHAGOCYTOTIC METABOLIC CHANGES OF LEUKOCYTES BY AN INTRACELLULAR CALCIUM-ANTAGONIST 8-(N,N-DIETHYLAMINO)-OCTYL-3,4,5-TRIMETHOXYBENZOATE

被引：73

作者：

MATSUMOTO, T

TAKESHIGE, K

MINAKAMI, S

机构：

[1] Department of Biochemistry, Kyushu University School of Medicine, Fukuoka

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91503-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of calcium in regulating the activity of leukocytes to generate and release superoxide was studied by using an intracellular calcium-antagonist, 8-(N,N-diethylamino)-octyl-3,4,5-trimethoxybenzoate. The antagonist inhibited the release of superoxide anions induced by a calcium-ionophore A23187 and the inhibition was relieved by the addition of calcium ions. The release induced by cytochalasin D or by the ingestion of bacteria was similarly inhibited by the calcium-antagonist. The result supports the hypothesis that an intracellular translocation of calcium is regulating the phagocytotic metabolic activity of leukocytes. The release of granule enzymes induced by the ionophore was also inhibited by the calcium antagonist. © 1979.

引用

收藏

页码：974 / 979

页数：6

相关论文

共 18 条

[1] BIOLOGICAL DEFENSE MECHANISMS - PRODUCTION BY LEUKOCYTES OF SUPEROXIDE A POTENTIAL BACTERICIDAL AGENT [J].

BABIOR, BM ;

KIPNES, RS ;

CURNUTTE, JT .

JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (03) :741-744

[2]

BERGMEYER HU, 1974, METHOD ENZYMAT AN, P574

[3] BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF AZUROPHIL AND SPECIFIC GRANULES OF HUMAN NEUTROPHILIC POLYMORPHONUCLEAR LEUKOCYTES [J].

BRETZ, U ;

BAGGIOLINI, M .

JOURNAL OF CELL BIOLOGY, 1974, 63 (01) :251-269

[4] INHIBITION OF PLATELET SECRETION BY AN ANTAGONIST OF INTRACELLULAR CALCIUM [J].

CHARO, IF ;

FEINMAN, RD ;

DETWILER, TC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 72 (04) :1462-1467

[5] SUPEROXIDE PRODUCTION BY DIGITONIN-STIMULATED GUINEA-PIG GRANULOCYTES - EFFECTS OF N-ETHYL MALEIMIDE, DIVALENT-CATIONS, AND GLYCOLYTIC AND MITOCHONDRIAL INHIBITORS ON ACTIVATION OF SUPEROXIDE GENERATING SYSTEM [J].

COHEN, HJ ;

CHOVANIEC, ME .

JOURNAL OF CLINICAL INVESTIGATION, 1978, 61 (04) :1088-1096

[6] STIMULATION OF INSULIN RELEASE BY CALCIUM [J].

DEVIS, G ;

SOMERS, G ;

MALAISSE, WJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 67 (02) :525-529

[7] APPEARANCE OF HYDROLASE RICH GRANULES IN HUMAN LYMPHOCYTES INDUCED BY PHYTOHEMAGGLUTININ AND ANTIGENS [J].

HIRSCHHORN, R ;

HIRSCHHORN, K ;

WEISSMANN, G .

BLOOD-THE JOURNAL OF HEMATOLOGY, 1967, 30 (01) :84-+

[8] STUDIES OF METABOLIC ACTIVITY OF LEUKOCYTES FROM PATIENTS WITH A GENETIC ABNORMALITY OF PHAGOCYTIC FUNCTION [J].

HOLMES, B ;

PAGE, AR ;

GOOD, RA .

JOURNAL OF CLINICAL INVESTIGATION, 1967, 46 (09) :1422-&

[9] BIOCHEMICAL ASPECTS OF PHAGOCYTOSIS [J].

IYER, GY ;

ISLAM, MF ;

QUASTEL, JH .

NATURE, 1961, 192 (480) :535-&

[10] PHARMACOLOGICAL EVALUATION OF A NEW CA++ ANTAGONIST, 8-(N,N-DIETHYLAMINO)OCTYL 3,4,5-TRIMETHOXYBENZOATE HYDROCHLORIDE (TMB-8) - STUDIES IN SKELETAL-MUSCLES [J].

MALAGODI, MH ;

CHIOU, CY .

PHARMACOLOGY, 1974, 12 (01) :20-31