PRODUCTION OF ALKALINE PROTEASE BY A NEW ASPERGILLUS-FLAVUS ISOLATE UNDER SOLID-SUBSTRATE FERMENTATION CONDITIONS FOR USE AS A DEPILATION AGENT

被引:119
作者
MALATHI, S
CHAKRABORTY, R
机构
关键词
D O I
10.1128/AEM.57.3.712-716.1991
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of alkaline protease by an Aspergillus flavus strain isolated in our laboratory by solid-substrate fermentation for use as a depilation agent and the influence of various factors on enzyme production are reported. The optimum conditions for maximum production were a growth temperature of 32-degrees-C, 63% substrate moisture, and a growth period of 48 h. Enrichment with corn steep liquor or Casitone increased productivity. Scaling-up experiments indicated that flask-scale results could be reproduced at 1 and 30 kg of substrate. The enzyme preparation exhibited maximum activity at both pH 7.5 and pH 9.5. The use of this enzyme as a depilation agent was confirmed by experiments in a tannery.
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页码:712 / 716
页数:5
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