CONSTRUCTION OF AN ESCHERICHIA-COLI-K-12 STRAIN DELETED FOR MANGANESE AND IRON SUPEROXIDE-DISMUTASE GENES AND ITS USE IN CLONING THE IRON SUPEROXIDE-DISMUTASE GENE OF LEGIONELLA-PNEUMOPHILA

被引：41

作者：

STEINMAN, HM

机构：

[1] Albert Einstein College of Medicine, Department of Biochemistry, Bronx, 10461, NY

来源：

MOLECULAR AND GENERAL GENETICS | 1992年 / 232卷 / 03期

关键词：

SUPEROXIDE DISMUTASE; DELETION MUTANTS; ESCHERICHIA-COLI; LEGIONELLA-PNEUMOPHILA;

D O I：

10.1007/BF00266247

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismutases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.

引用

页码：427 / 430

页数：4

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