COOMASSIE BLUE PROTEIN DYE-BINDING ASSAYS MEASURE FORMATION OF AN INSOLUBLE PROTEIN DYE COMPLEX

被引：36

作者：

MARSHALL, T

WILLIAMS, KM

机构：

[1] Biochemistry Research Laboratory, School of Pharmaceutical and Chemical Sciences, Sunderland Polytechnic, Sunderland, SR2 7EE, Galen Building, Green Terrace

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 204卷 / 01期

关键词：

D O I：

10.1016/0003-2697(92)90147-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The solubility of the protein-Coomassie brilliant blue (CBB) complex formed upon Bradford (Anal. Biochem. 72, 248-254, 1976) or Sedmak and Grossberg (Anal. Biochem. 79, 544-552, 1977) protein assay has been investigated by centrifugation or filtration of the assay mix within 10 min of adding dye reagent. The results show complete loss of color yield in the respective supernates and filtrates. This indicates that the protein-CBB complexes are insoluble at the time of absorbance measurement. Protein solubility in the dye reagent may dictate the relative response of the assay to an individual protein and the requirement for macromolecular structure. © 1992.

引用

页码：107 / 109

页数：3

共 7 条

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