MOLECULAR-CLONING AND EXPRESSION OF A CDNA-ENCODING A RECEPTOR FOR PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP)

被引：124

作者：

MORROW, JA ^{[1
]}

LUTZ, EM ^{[1
]}

WEST, KM ^{[1
]}

FINK, G ^{[1
]}

HARMAR, AJ ^{[1
]}

机构：

[1] UNIV EDINBURGH,DEPT PHARMACOL,MRC,BRAIN METAB UNIT,EDINBURGH EH8 9YL,MIDLOTHIAN,SCOTLAND

来源：

FEBS LETTERS | 1993年 / 329卷 / 1-2期

关键词：

PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE (PACAP); G-PROTEIN-LINKED RECEPTOR; VASOACTIVE INTESTINAL PEPTIDE; RAT PITUITARY; RAT OLFACTORY BULB; CDNA CLONING;

D O I：

10.1016/0014-5793(93)80202-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced a novel cDNA (RPR7) encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP). RPR7 was identified by PCR of rat pituitary cDNA, and full-length clones were isolated from a rat olfactory bulb cDNA library. When expressed in COS cells, RPR7 was functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP) in response to stimulation by PACAP-38, PACAP-27, vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI). The order of potency of these ligands was PACAP-38 is similar to PACAP-27 > VIP > PHI, suggesting that the receptor corresponds to the pharmacologically characterised PACAP Type I receptor.

引用

页码：99 / 105

页数：7

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