SEPARATION OF MYOSIN HEAVY-CHAIN ISOFORMS IN RAT SKELETAL-MUSCLES BY GRADIENT SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS

被引：83

作者：

SUGIURA, T ^{[1
]}

MURAKAMI, N ^{[1
]}

机构：

[1] YAMAGUCHI UNIV,SCH MED,DEPT PHYSIOL,UBE,YAMAGUCHI 755,JAPAN

来源：

BIOMEDICAL RESEARCH-TOKYO | 1990年 / 11卷 / 02期

关键词：

D O I：

10.2220/biomedres.11.87

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

This study was conducted to investigate the electrophoretic condition for the separation of a novel myosin heavy chain isoform, designated as HCIId, in rat skeletal muscles by gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis. HCIId was separated on a polyacrylamide (5-8%)/glycerol (30-40%) gradient gel when electrophoresis was performed at 150V for about 18 h at 8°C. HCIId was observed in most fast-twitch muscles. Of those muscles, the deep portion of the masseter muscle contained the most abundant HCIId and one of the masseter muscles contained the neonatal-type MHC. © 1990, Biomedical Research Press. All rights reserved.

引用

页码：87 / 91

页数：5

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