RAT SERUM RIBONUCLEASE

被引：13

作者：

UMEDA, T

MORIYAMA, T

OURA, H

TSUKADA, K

机构：

[1] Department of Biochemistry, Drug Research Institute, Faculty of Pharmaceutical Science, Gofuku, Toyama

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 171卷 / 02期

关键词：

D O I：

10.1016/0005-2744(69)90159-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat serum ribonuclease has been purified about 6000-fold. The optimal pH of this enzyme was 7-8. The purified enzyme was rather heat labile at pH 7.6. The Km of this enzyme is 30% higher than that of the crystalline bovine pancreatic ribonuclease (polyribonucleotide 2-oligonucleotide-transferase, EC 2.7.7.16). No stimulatory or inhibitory effect on this enzyme was observed with EDTA, KCl, MgCl2. p-chloromercuribenzoinc acid, or mercaptoethanol. © 1969.

引用

页码：260 / &

共 12 条

[1] ALEXANDROWICZ J, 1955, SANG, V26, P212
[2] COELHO E, 1925, BIOCHEM Z, V161, P71
[3] JONES W, 1920, NUCLEIC ACIDS THEIR, P91
[4] LOWRY OH, 1951, J BIOL CHEM, V193, P265
[5] METAIS P, 1955, B SOC CHIM BIOL PARI, V37, P99
[6] MORIYAMA T, TO BE PUBLISHED
[7] COLUMN CHROMATOGRAPHY OF PROTEINS - SUBSTITUTED CELLULOSES
PETERSON, EA
SOBER, HA
[J]. METHODS IN ENZYMOLOGY, 1962, 5 : 3 - 27
[8] CHARACTERISTICS OF RAT AND GUINEA PIG SERUM RIBONUCLEASE
RABINOVITCH, M
DOHI, SR
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1957, 70 (01) : 239 - 247
[9] ROTH JS, 1952, J BIOL CHEM, V196, P489
[10] ISOLATION AND PHYSICAL PROPERTIES OF RIBOSOMAL RIBONUCLEIC ACID OF ESCHERICHIA COLI
STANLEY, WM
BOCK, RM
[J]. BIOCHEMISTRY, 1965, 4 (07) : 1302 - &

← 1 2 →