A TRANSPOSON-RELATED PALINDROMIC REPETITIVE SEQUENCE FROM C-ELEGANS

被引:26
作者
DREYFUS, DH
EMMONS, SW
机构
[1] Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461
关键词
D O I
10.1093/nar/19.8.1871
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A family of transposon-like sequences in the C. elegans genome is described. This family, termed the Tc6 family, consists mostly of conserved, 1.6 kb elements. Four Tc6 elements or partial elements have been cloned and the DNA sequences of three were determined. One appears to be a complete element of 1603 nucleotides, consisting of a palindrome of 765 nucleotides, with a central, non-palindromic region of 73 nucleotides. Another has an identical structure except for an internal deletion. A third is a partial element terminating at a probable internal restriction site used for cloning. A fourth clone contained portions of the Tc6 sequence juxtaposed to non-Tc6 sequences. All C. elegans strains examined contain 20-30 Tc6 elements. The ends of Tc6 elements are conserved and have sequence similarity to the ends of C. elegans transposons Tc1 and Tc3. The ends of Tc6 elements also have sequence similarity to the heptamer portion of the immunoglobulin and T-cell receptor recombination signal sequence, raising the possibility of wide phylogenetic conservation of the recombination mechanism. Tc6 elements also share sequence motifs with plant-pathogenic viroid RNA's, possibly indicative of a Tc6 RNA replicative phase.
引用
收藏
页码:1871 / 1877
页数:7
相关论文
共 41 条
  • [1] 2 PAIRS OF RECOMBINATION SIGNALS ARE SUFFICIENT TO CAUSE IMMUNOGLOBULIN-V-(D)-J JOINING
    AKIRA, S
    OKAZAKI, K
    SAKANO, H
    [J]. SCIENCE, 1987, 238 (4830) : 1134 - 1138
  • [2] Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210
  • [3] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM
    BOLIVAR, F
    RODRIGUEZ, RL
    GREENE, PJ
    BETLACH, MC
    HEYNEKER, HL
    BOYER, HW
    CROSA, JH
    FALKOW, S
    [J]. GENE, 1977, 2 (02) : 95 - 113
  • [4] BRENNER S, 1974, GENETICS, V77, P71
  • [5] THE TRANSPOSABLE ELEMENT UHU FROM HAWAIIAN DROSOPHILA - MEMBER OF THE WIDELY DISPERSED CLASS OF TC1-LIKE TRANSPOSONS
    BREZINSKY, L
    WANG, GVL
    HUMPHREYS, T
    HUNT, J
    [J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (08) : 2053 - 2059
  • [6] DISTINCT CHARACTERISTICS OF LOOP SEQUENCES OF 2 DROSOPHILA FOLDBACK TRANSPOSABLE ELEMENTS
    BRIERLEY, HL
    POTTER, SS
    [J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (02) : 485 - 500
  • [7] COLLINS J, 1989, GENETICS, V121, P47
  • [8] ACTIVATION OF A TRANSPOSABLE ELEMENT IN THE GERM LINE BUT NOT THE SOMA OF CAENORHABDITIS-ELEGANS
    COLLINS, J
    SAARI, B
    ANDERSON, P
    [J]. NATURE, 1987, 328 (6132) : 726 - 728
  • [9] TOWARD A PHYSICAL MAP OF THE GENOME OF THE NEMATODE CAENORHABDITIS-ELEGANS
    COULSON, A
    SULSTON, J
    BRENNER, S
    KARN, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (20) : 7821 - 7825
  • [10] GENOME LINKING WITH YEAST ARTIFICIAL CHROMOSOMES
    COULSON, A
    WATERSTON, R
    KIFF, J
    SULSTON, J
    KOHARA, Y
    [J]. NATURE, 1988, 335 (6186) : 184 - 186