MODIFICATION OF THE QUANTITATIVE DNA ASSAY USING ADRIAMYCIN

被引：2

作者：

DALBOW, DG

BARTUSKA, BM

机构：

[1] Department of Pathology, Colorado State University, Fort Collins

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90772-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modification of the adriamycin quantitative assay for DNA is presented which is sensitive to 1-20 .mu.g DNA. The assay is simple, rapid and highly reproducible but, contrary to previous claims, not specific for DNA. RNA interferes with the assay; protein does not. The mechanism and sensitivity of the assay is interpreted with reference to published data on adriamycin DNA binding.

引用

页码：559 / 562

页数：4

共 9 条

[1] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID
BURTON, K
[J]. BIOCHEMICAL JOURNAL, 1956, 62 (02) : 315 - 323
[2] ON PHYSICO-CHEMICAL INTERACTIONS BETWEEN DAUNOMYCIN AND NUCLEIC ACIDS
CALENDI, E
DIMARCO, A
REGGIANI, M
SCARPINATO, B
VALENTINI, L
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 103 (01) : 25 - +
[3] GOODMAN MF, 1977, J BIOL CHEM, V252, P2670
[4] USE OF ANTI-TUMOUR ANTIBIOTICS FOR SIMPLE QUANTITATIVE ASSAYS FOR DNA
HILL, BT
[J]. ANALYTICAL BIOCHEMISTRY, 1976, 70 (02) : 635 - 638
[5] DETERMINATION OF NUCLEIC ACIDS IN BIOLOGICAL MATERIALS - A REVIEW
HUTCHISON, W
MUNRO, HN
[J]. ANALYST, 1961, 86 (102) : 768 - &
[6] PHYSICOCHEMICAL PROPERTIES OF COMPLEXES BETWEEN DEOXYRIBONUCLEIC ACID AND ANTIBIOTICS WHICH AFFECT RIBONUCLEIC ACID SNYTHESIS (ACTINOMYCIN DAUNOMYCIN CINERUBIN NOGALAMYCIN CHROMOMYCIN MITHRAMYCIN AND OLIVOMYCIN)
KERSTEN, W
KERSTEN, H
SZYBALSKI, W
[J]. BIOCHEMISTRY, 1966, 5 (01) : 236 - +
[7] MUNRO H. N., 1966, METHOD BIOCHEM ANAL, V14, P113
[8] SPECTROSCOPIC STUDIES OF INTERACTION OF DAUNOMYCIN WITH TRANSFER-RNA
SHAFER, RH
[J]. BIOCHEMICAL PHARMACOLOGY, 1977, 26 (18) : 1729 - 1734
[9] INTERACTION OF DAUNOMYCIN AND ITS DERIVATIVES WITH DNA
ZUNINO, F
ZACCARA, A
GAMBETTA, R
DIMARCO, A
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 277 (03) : 489 - &

← 1 →