FLUORESCENCE POLARIZATION IMMUNOASSAY FOR CORTISOL

被引：37

作者：

KOBAYASHI, Y

AMITANI, K

WATANABE, F

MIYAI, K

机构：

[1] KOBE WOMENS COLL PHARM,CLIN CHEM LAB,HIGASHINADA KU,KOBE,JAPAN

[2] OSAKA UNIV HOSP,CENT CLIN INVESTIGAT LAB,FUKUSHIMA KU,OSAKA,FUKUSHIMA 553,JAPAN

来源：

CLINICA CHIMICA ACTA | 1979年 / 92卷 / 02期

关键词：

D O I：

10.1016/0009-8981(79)90119-0

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A fluorescence polarization immunoassay for serum cortisol was established using cortisol 21-amine which was readily coupled with fluorescein isothiocyanate. The proposed method is sufficiently sensitive, reliable, specific and simple for routine determination of serum cortisol. The assay is rapid, without separation of antibody-bound and free ligands. The minimal amount of cortisol detected was 1.5 ng/tube and the measurable range was from 1.5 to 100 μg/dl. There was a good correlation between values obtained from radioimmunoassay and the proposed method. © 1979.

引用

页码：241 / 247

页数：7

共 10 条

[1] IMMUNO-ENZYMATIC ASSAY OF CORTISOL USING ESCHERICHIA-COLI BETA-GALACTOSIDASE AS LABEL [J].