PROMOTER-UPSTREAM ACTIVATOR SEQUENCES ARE REQUIRED FOR EXPRESSION OF THE XYLS GENE AND UPPER-PATHWAY OPERON ON THE PSEUDOMONAS TOL PLASMID

被引：24

作者：

HOLTEL, A ^{[1
]}

ABRIL, MA ^{[1
]}

MARQUES, S ^{[1
]}

TIMMIS, KN ^{[1
]}

RAMOS, JL ^{[1
]}

机构：

[1] NATL CTR BIOTECHNOL,GBF,W-3300 BRAUNSCHWEIG,GERMANY

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 09期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb02066.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stimulation of transcription from the Pseudomonas TOL plasmid xylS gene promoter (Ps) and the upperpathway operon promoter (Pu) is dependent on the positive regulator protein XylR activated by an effector molecule such as 3‐cholorotoluene, and on RpoN, an RNA polymerase sigma factor. Mutational analysis of the Ps and Pu promoters showed that upstream activator sequences located between ‐110 and ‐218bp upstream of the main transcription initiation point are required for regulated expression from these promoters. A search for homologous nucleotide sequences in the ‐110to ‐218bp region in Pu and Ps revealed conserved sequences that may act as putative recognition sequences for the XylR protein. Ps and Pu exhibit another well‐conserved region at around 50 bp, which is homologous to corresponding sites in other RpoN‐dependent promoters and may constitute a binding site for integration host factor (IHF). Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1551 / 1556

页数：6

共 38 条

[1] REGULATOR AND ENZYME SPECIFICITIES OF THE TOL PLASMID-ENCODED UPPER PATHWAY FOR DEGRADATION OF AROMATIC-HYDROCARBONS AND EXPANSION OF THE SUBSTRATE RANGE OF THE PATHWAY [J].