IGFBP-1, AN INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN, IS A CELL-GROWTH INHIBITOR

被引：71

作者：

LIU, L ^{[1
]}

BRINKMAN, A ^{[1
]}

BLAT, C ^{[1
]}

HAREL, L ^{[1
]}

机构：

[1] ERASMUS UNIV,3000 DR ROTTERDAM,NETHERLANDS

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91470-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel cell growth inhibitor, IDF45 (inhibitory diffusible factor), was recently purified to apparent homogeneity. It is a bifunctional molecule: able to bind Insulin like growth factor (IGF) and to 100 % inhibit DNA synthesis stimulated by serum in fibroblasts. It was of interest to verify whether other members of the IGF-binding protein (IGFBP) family show the same bifunctional growth inhibitory properties. In this paper we show that purified IGFBP-1 derived from amniotic fluid is a cell growth inhibitor. In chick embryo fibroblasts, it inhibited DNA synthesis stimulated by serum. However the stimulation was maximally 60 % inhibited and half of the inhibition was observed with 100ng/ml IGFBP-1. So the specific activity of IGFBP-1 is lower than that of IDF45. IGFBP-1 also reversibly prevented the CEF growth. In the same cells IGFBP-1 inhibited DNA synthesis stimulated by IGF-I. We demonstrated that the same protein IGFBP-1 is able to inhibit DNA synthesis stimulated by serum and by IGF-I. The possibility that IGFBP-1 is a bifunctional molecule is discussed. © 1991.

引用

页码：673 / 679

页数：7

共 15 条

[1] INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN (IGF-BP) INHIBITION OF GRANULOSA-CELL FUNCTION - EFFECT ON CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE, DEOXYRIBONUCLEIC-ACID SYNTHESIS, AND COMPARISON WITH THE EFFECT OF AN IGF-I ANTIBODY [J].