PHOSPHOLIPASE-C-GAMMA-1 ASSOCIATION WITH CD3 STRUCTURE IN T-CELLS

被引：63

作者：

DASGUPTA, JD

GRANJA, C

DRUKER, B

LIN, LL

YUNIS, EJ

RELIAS, V

机构：

[1] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV CELLULAR & MOLEC BIOL,BOSTON,MA 02115

[2] GENET INST,CAMBRIDGE,MA 02140

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 1992年 / 175卷 / 01期

关键词：

D O I：

10.1084/jem.175.1.285

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recently, we and others have reported tyrosine phosphorylation of phospholipase C-gamma-1 (PLC-gamma-1) enzyme after CD3 activation of T cells, and have proposed that PLC-gamma-1 mediates signal transduction through the T cell receptor (TCR/CD3). Here, using immunoblotting and immune complex PLC assays, we show that CD3 stimulation of Jurkat cells induces the association of PLC-gamma-1 enzyme with CD3 complex. PLC activity is also found to co-precipitate with the CD3-zeta-chain from activated cells. In addition, in vitro PLC assays show that CD3 activation leads to about 10-fold stimulation of PLC-gamma-1 activity. These results, along with the observation that Jurkat cells preferentially express PLC-gamma-1, indicate that PLC-gamma-1 participates in CD3 signaling.

引用

页码：285 / 288

页数：4

共 21 条

[1] INHIBITION OF T-CELL ANTIGEN RECEPTOR-MEDIATED TRANSMEMBRANE SIGNALING BY PROTEIN KINASE-C ACTIVATION [J].