CELLULAR-LOCALIZATION OF THE ESCHERICHIA-COLI SPOT PROTEIN

被引：36

作者：

GENTRY, DR

CASHEL, M

机构：

[1] Laboratory of Molecular Genetics, NICHHD, National Institutes of Health, Bethesda

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 13期

关键词：

D O I：

10.1128/jb.177.13.3890-3893.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The SpoT protein of Escherichia coli serves as a source of degradation as well as an apparent source of synthesis of (p)ppGpp. Since the subcellular localization of SpoT might be a clue to its function, we have used SpoT-specific antisera to analyze cell extracts fractionated on sucrose gradients. We find that the SpoT protein is not bound to ribosomes or to either inner or outer membrane fractions. Although the SpoT protein is found in large aggregates, its localization is probably cytosolic

引用

页码：3890 / 3893

页数：4

共 31 条

[1] CLONING THE SPOT GENE OF ESCHERICHIA-COLI - IDENTIFICATION OF THE SPOT GENE-PRODUCT [J].