ISOLATION AND CHARACTERIZATION OF A TOBACCO CDNA CLONE ENCODING A PUTATIVE MAP KINASE

被引:65
作者
WILSON, C
ELLER, N
GARTNER, A
VICENTE, O
HEBERLEBORS, E
机构
[1] UNIV VIENNA,VIENNA BIOCTR,INST MICROBIOL & GENET,DR BOHR GASSE 9,A-1030 VIENNA,AUSTRIA
[2] VIENNA BIOCTR,INST MOLEC PATHOL,A-1030 VIENNA,AUSTRIA
关键词
CELL CYCLE; MAP KINASES; NICOTIANA-TABACUM; POLLEN DEVELOPMENT; POLLEN EMBRYOGENESIS; STARVATION;
D O I
10.1007/BF00019302
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated and sequenced a MAP (mitogen-activated protein) kinase-type cDNA from a tobacco (Nicotiana tabacum L.) cell suspension cDNA library by screening with a PCR fragment amplified from the same library with oligonucleotide primers corresponding to two sequences conserved in yeast and animal MAP kinases. The tobacco sequence, ntf3, shows 45-54% identity to various members of the MAP kinase family at the protein level. Northern experiments showed that ntf3 is expressed in all tobacco tissues tested, including pollen isolated at different developmental stages. Southern analysis indicated that, as in other organisms, there is a family of MAP kinase genes in tobacco. In complementary tests, ntf3 could not substitute the yeast MAP kinase genes fus3 and kss1.
引用
收藏
页码:543 / 551
页数:9
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