PHOTOSENSITIZATION BY HYPERICIN - ELECTRON-SPIN-RESONANCE (ESR) EVIDENCE FOR THE FORMATION OF SINGLET OXYGEN AND SUPEROXIDE ANION-RADICALS IN AN IN-VITRO MODEL

被引：90

作者：

HADJUR, C ^{[1
]}

JEUNET, A ^{[1
]}

JARDON, P ^{[1
]}

机构：

[1] UNIV GRENOBLE 1,LEDSS,F-38041 GRENOBLE,FRANCE

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1994年 / 26卷 / 01期

关键词：

HYPERICIN; ESR; SINGLET OXYGEN; PHOTOSENSITIZATION; SUPEROXIDE ANION RADICALS;

D O I：

10.1016/1011-1344(94)85037-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of hypericin (HYP) to photoreact by type I and type II mechanisms was investigated in egg yolk lecithin (EYL) liposomes as a model membrane system. On visible irradiation in aerobic conditions at pH 7, the formation of singlet oxygen and superoxide anion radicals was demonstrated unequivocally using electron spin resonance (ESR) spectrometry with the spin traps 2,2,6,6-tetramethyl-4-piperidone (TEMP) and 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). We conclude from our results that HYP in EYL liposome membranes appears to act through both singlet oxygen and electron transfer mechanisms.

引用

页码：67 / 74

页数：8

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