GRADIENT ELUTION IN LIQUID-CHROMATOGRAPHY .10. RETENTION CHARACTERISTICS IN REVERSED-PHASE GRADIENT ELUTION CHROMATOGRAPHY

被引：81

作者：

JANDERA, P

CHURACEK, J

SVOBODA, L

机构：

[1] Department of Analytical Chemistry, University of Chemical Technology, Pardubice

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 174卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)87035-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The reversed-phae chromatographic behaviour of selected barbiturates, alkaloids and substituted alkyluracils on a C18 bonded phase column was investigated in both isocratic and gradient elution experiments using a binary mobile phase composed of water and methanol. Experimental capacity ratios of sample compounds under isocratic conditions fitted well with the linear relationships of log k′ versus concentration of methanol in the mobile phase. The approach for the calculation of retention volumes and peak widths in gradient elution chromatography presented earlier was extended to reversed-phase chromatography and verified experimentally. The agreement between the experimental and theoretically predicted values was satisfactory. © 1979.

引用

页码：35 / 50

页数：16

共 19 条

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