CHROMOSOMAL REGION OF THE CYSTIC-FIBROSIS GENE IN YEAST ARTIFICIAL CHROMOSOMES - A MODEL FOR HUMAN GENOME MAPPING

被引：234

作者：

GREEN, ED ^{[1
]}

OLSON, MV ^{[1
]}

机构：

[1] WASHINGTON UNIV, SCH MED, HOWARD HUGHES MED INST, ST LOUIS, MO 63110 USA

来源：

SCIENCE | 1990年 / 250卷 / 4977期

关键词：

D O I：

10.1126/science.2218515

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A general strategy for cloning and mapping large regions of human DNA with yeast artificial chromosomes (YAC's) is described. It relies on the use of the polymerase chain reaction to detect DNA landmarks called sequence-tagged sites (STS's) within YAC clones. The method was applied to the region of human chromosome 7 containing the cystic fibrosis (CF) gene. Thirty YAC clones from this region were analyzed, and a contig map that spans more than 1,500,000 base pairs was assembled. Individual YAC's as large as 790 kilobase pairs and containing the entire CF gene were constructed in vivo by meiotic recombination in yeast between pairs of overlapping YAC's.

引用

页码：94 / 98

页数：5

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