UROPORPHYRINOGEN-III SYNTHASE - STUDIES ON ITS MECHANISM OF ACTION, MOLECULAR-BIOLOGY AND BIOCHEMISTRY

被引：12

作者：

CROCKETT, N ^{[1
]}

ALEFOUNDER, PR ^{[1
]}

BATTERSBY, AR ^{[1
]}

ABELL, C ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,CHEM LAB,LENSFIELD RD,CAMBRIDGE CB2 1EW,ENGLAND

来源：

TETRAHEDRON | 1991年 / 47卷 / 31期

关键词：

D O I：

10.1016/S0040-4020(01)86492-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A review is given of studies on the mechanism of action of uroporphyrinogen III synthase (cosynthetase, EC 4.2.1.75) based on synthesis of an inhibitory spiro-lactam. HemD, the Escherichia coli gene coding for uroporphyrinogen III synthase has been cloned and overexpressed at levels sixteen fold higher than in wild type E. coli. Evidence for hemD being part of an operon is reviewed. Uroporphyrinogen III synthase, which has been purified approx. 6000 fold, shows M(r) 28000 under denaturing conditions and has a pH optimum of 8.0 +/- 0.2. The results from chemical modification of the enzyme point to the presence of arginine and lysine residues at or close to the active site.

引用

页码：6003 / 6014

页数：12

共 38 条

[1] GENERATION OF A DETAILED PHYSICAL AND GENETIC-MAP OF THE ILV-METE-UDP REGION OF THE ESCHERICHIA-COLI CHROMOSOME [J].

ALDEA, M ;

MAPLES, VF ;

KUSHNER, SR .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 200 (03) :427-438

[2] THE SEQUENCE OF HEMC, HEMD AND 2 ADDITIONAL ESCHERICHIA-COLI GENES [J].

ALEFOUNDER, PR ;

ABELL, C ;

BATTERSBY, AR .

NUCLEIC ACIDS RESEARCH, 1988, 16 (20) :9871-9871

[3] PURIFICATION AND PROPERTIES OF UROPORPHYRINOGEN-III SYNTHASE (CO-SYNTHASE) FROM AN OVERPRODUCING RECOMBINANT STRAIN OF ESCHERICHIA-COLI K-12 [J].

ALWAN, AF ;

MGBEJE, BIA ;

JORDAN, PM .

BIOCHEMICAL JOURNAL, 1989, 264 (02) :397-402

[4] LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8 [J].

BACHMANN, BJ .

MICROBIOLOGICAL REVIEWS, 1990, 54 (02) :130-197

[5] BIOSYNTHESIS OF PORPHYRINS AND RELATED MACROCYCLES .20. PURIFICATION OF DEAMINASE AND STUDIES ON ITS MODE OF ACTION [J].

BATTERSBY, AR ;

FOOKES, CJR ;

MATCHAM, GWJ ;

MCDONALD, E ;

HOLLENSTEIN, R .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1983, (12) :3031-3040

[6] BIOSYNTHESIS OF PORPHYRINS AND RELATED MACROCYCLES .17. CHEMICAL AND ENZYMIC TRANSFORMATION OF ISOMERIC AMINOMETHYLBILANES INTO UROPORPHYRINOGENS - PROOF THAT UNREARRANGED BILANE IS THE PREFERRED ENZYMIC SUBSTRATE AND DETECTION OF A TRANSIENT INTERMEDIATE [J].

BATTERSBY, AR ;

FOOKES, CJR ;

GUSTAFSONPOTTER, KE ;

MCDONALD, E ;

MATCHAM, GWJ .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1982, (10) :2413-2426

[7]

BATTERSBY AR, 1982, J CHEM SOC PERK T 1, P2427, DOI 10.1039/p19820002427

[8] BIOSYNTHESIS OF PORPHYRINS AND RELATED MACROCYCLES .29. SYNTHESIS AND CHEMISTRY OF 2,2-DISUBSTITUTED 2H-PYRROLES (PYRROLENINES) [J].

BATTERSBY, AR ;

BAKER, MG ;

BROADBENT, HA ;

FOOKES, CJR ;

LEEPER, FJ .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1987, (09) :2027-2048

[9]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[10]

CASSIDY MC, UNPUB

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