THE FMRFAMIDE-LIKE NEUROPEPTIDE AF2 IS PRESENT IN THE PARASITIC NEMATODE HAEMONCHUS-CONTORTUS

被引：37

作者：

KEATING, CD

HOLDENDYE, L

THORNDYKE, MC

WILLIAMS, RG

MALLETT, A

WALKER, RJ

机构：

[1] UNIV SOUTHAMPTON,DEPT PHYSIOL & PHARMACOL,SOUTHAMPTON SO16 7PX,HANTS,ENGLAND

[2] UNIV LONDON,ROYAL HOLLOWAY & BEDFORD NEW COLL,SCH BIOL SCI,EGHAM TW20 0EX,SURREY,ENGLAND

[3] UNITED MED & DENT SCH,ST THOMAS HOSP,ST JOHNS INST DERMATOL,LONDON SE1 7EH,ENGLAND

来源：

PARASITOLOGY | 1995年 / 111卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

HAEMONCHUS CONTORTUS; AF2; FMRFAMIDE-RELATED PEPTIDES; IMMUNOCYTOCHEMISTRY; NEUROPEPTIDES;

D O I：

10.1017/S0031182000066026

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Peptides belonging to the FMRF amide family are widely distributed amongst invertebrates. We report here on the isolation of the FMRF amide neuropeptide AF2 (Lys-His-Glu-Tyr-Leu-Arg-Phe-NH2) from thr parasitic nematode Haemonchus contortus. Immunocytochemical techniques showed that FMRF amide-like material was distributed in several regions of these organisms including nerve cords and cell bodies of the central nervous system. AF2 was isolated using a method that employed 6 steps oi reverse-phase HPLC. The concentration oi AF2 in this organism was approximately 30 pmol/g of nematode.

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页码：515 / 521

页数：7

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