SCALLOP STRIATED AND SMOOTH-MUSCLE MYOSIN HEAVY-CHAIN ISOFORMS ARE PRODUCED BY ALTERNATIVE RNA SPLICING FROM A SINGLE-GENE

被引:46
作者
NYITRAY, L
JANCSO, A
OCHIAI, Y
GRAF, L
SZENTGYORGYI, AG
机构
[1] BRANDEIS UNIV, DEPT BIOL, WALTHAM, MA 02254 USA
[2] EOTVOS LORAND UNIV, DEPT BIOCHEM, H-1088 BUDAPEST, HUNGARY
关键词
CATCH MUSCLE; POLYMERASE CHAIN REACTION;
D O I
10.1073/pnas.91.26.12686
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We report here that the catch and striated adductor muscle myosin heavy chain (MHC) isoforms of scallop (Argopecten irradians, previously Aequipecten irradians) are generated by alternative RNA splicing from a single gene. Scallop catch muscle cDNA and genomic DNA were amplified by PCR using primers based on the previously sequenced scallop striated muscle MHC cDNA. Mapping of the exon/intron borders acid sequencing of a full-length catch muscle MHC in overlapping fragments revealed that the 24-kb gene encodes the MHC polypeptide in 27 exons and that four sets of tandem exon pairs are alternatively spliced into a striated and a catch MHC isoform. An additional alternative exon was identified in catch cDNA and is apparently spliced into a minor MHC isoform. The striated muscle-specific isoform is not expressed in other tissues, whereas the catch-type isoforms were also detected in various smooth muscles, but not in the striated one. Of the alternative exons, exons 5 and 6 encode part of the ATP-binding region and the 25-kDa/50-kDa proteolytic junction; exon 13 encodes part of one of the actin-binding regions and extends to the active site; exon 20 encodes the middle of the rod hinge region; exon 26 in the striated-specific sequence starts with the stop codon, whereas the catch-specific exon codes for an additional 10 residues. Differences between the alternative exons presumably determine the lower ATPase activity of smooth muscle myosin, contribute to the different structure of the striated and smooth muscle thick filaments, and may also be important for the molecular mechanism of the catch phenomenon.
引用
收藏
页码:12686 / 12690
页数:5
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