COMPARATIVE-STUDY OF THE INVITRO SYNTHESIS OF PROSTAGLANDINS AND THROMBOXANES IN PLANTS BELONGING TO LILIACEAE FAMILY

被引:8
作者
ALI, M [1 ]
AFZAL, M [1 ]
HASSAN, RAH [1 ]
FARID, A [1 ]
BURKA, JF [1 ]
机构
[1] UNIV PRINCE EDWARD ISL,COLL VET,DEPT ANAT & PHYSIOL,CHARLOTTETOWN C1A 4P3,PEI,CANADA
来源
GENERAL PHARMACOLOGY | 1990年 / 21卷 / 03期
关键词
D O I
10.1016/0306-3623(90)90821-3
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1. 1. Homogenates of garlic (Allium sativum), onions (Allium cepa) and Allium porum were in vitro incubated with [14C]arachidonic acid. 2. 2. Separation of labelled prostaglandins and thromboxanes were accomplished by thin-layer chromatography (TLC) and the Rj values were compared with those of authentic standards. 3. 3. The prostaglandins identified were 6-keto-PGF1α, PGF2α, TXB2, PGE2 and PGD2. 4. 4. PGE2 and PGD2 were the major metabolites of arachidonic acid among all the members of the Liliaceae family studied. 5. 5. Garlic was found to have the highest capacity to metabolize the [14C]arachidonic acid into prostaglandins and thromboxanes. 6. 6. The synthesis of prostaglandins and thromboxanes, was inhibited by preincubation of homogenates with indomethacin or was completely destroyed by boiling the plant extract prior to incubation with arachidonic acid. This confirmed the presence of cyclooxygenase in these plants. © 1990.
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页码:273 / 276
页数:4
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