STABLE TRANSFORMATION OF THE MOSS PHYSCOMITRELLA-PATENS

被引：188

作者：

SCHAEFER, D

ZRYD, JP

KNIGHT, CD

COVE, DJ

机构：

[1] UNIV LAUSANNE,PHYTOGENET CELLULAIRE LAB,CH-1015 LAUSANNE,SWITZERLAND

[2] UNIV LEEDS,DEPT GENET,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 226卷 / 03期

关键词：

PHYSCOMITRELLA-PATENS; TRANSFORMATION;

D O I：

10.1007/BF00260654

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the stable transformation of Physcomitrella patens to either G418 or hygromycin B resistance following polyethylene glycol-mediated direct DNA uptake by protoplasts. The method described in this paper was used successfully in independent experiments carried out in our two laboratories. Transformation was assessed by the following criteria: selection of antibiotic-resistant plants, mitotic and meiotic stability of phenotypes after removal of selective pressure and stable transmission of the character to the offspring; Southern hybridisation analysis of genomic DNA to show integration of the plasmid DNA; segregation of the resistance gene following crosses with antibiotic-sensitive strains; and finally Southern hybridisation analysis of both resistant and sensitive progeny. In addition to stable transformants, a heterogeneous class of unstable transformants was obtained.

引用

页码：418 / 424

页数：7

共 33 条

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