MEASUREMENT OF INTRACELLULAR MAGNESIUM IN A VASCULAR SMOOTH-MUSCLE CELL-LINE USING A FLUORESCENT-PROBE

被引：29

作者：

SCHACHTER, M

GALLAGHER, KL

SEVER, PS

机构：

[1] Department of Clinical Pharmacology, St. Mary's Hospital, London

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1035卷 / 03期

关键词：

Calcium; Fluorescent probe; Magnesium; Vascular smooth muscle;

D O I：

10.1016/0304-4165(90)90103-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Until recently, direct measurement of intracellular free magnesium has been complex and difficult. However, fluorescent probes are now available, based on the same principle as well-established probes for free calcium. Using one such probe, mag-fura-2, we have estimated basal intracellular magnesium concentrations in the A7r5 rat vascular smooth muscle cell line. This level unaffected by numerous pharmacological manipulations, including agonist stimulation and depolarisation. The possible implications of these findings are discussed. © 1990.

引用

页码：378 / 380

页数：3

共 18 条

[1] MG-2+-CA-2+ INTERACTION IN CONTRACTILITY OF VASCULAR SMOOTH-MUSCLE - MG-2+ VERSUS ORGANIC CALCIUM-CHANNEL BLOCKERS ON MYOGENIC TONE AND AGONIST-INDUCED RESPONSIVENESS OF BLOOD-VESSELS [J].