PREPARATIVE SEPARATION OF BRAIN MITOCHONDRIAL MONO-AMINE OXIDASES

被引：16

作者：

MOSKVITINA, TA

KAMYSHANSKAYA, NS

GARISHVILI, TG

GORKIN, VZ

机构：

[1] Laboratory of Biogenic Amines and Other Nitro— genous Bases, Institute of Biological and Me, Dical Chemistry, Academy of Medical Sciences of the USSR, Moscow, USSR, Pogodinskaya Street

来源：

PREPARATIVE BIOCHEMISTRY | 1979年 / 9卷 / 02期

关键词：

D O I：

10.1080/00327487908061682

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method was developed for solubilization from-bo-vine brain stem mitochondria' fraction of monoamine oxidases deminating biogenic amines. Preparative separation of the monoamine oxidases, possessing different substrate specificities, was achieved by column chromatography on a biospecific adsorbent AH—Sepharose 4 B. The enzyme preparations thus obtained did not contain any detectable by disc—electrophoresis or isoelectrofocusing in polyacrylamide gels proteins which were devoid of the mo - noamine oxidase activity. © 1979, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：171 / 196

页数：26

共 31 条

[1] AKOPYAN ZI, 1971, INT J PROT RES, V3, P121
[2] BAUMANIS E A, 1978, Biokhimiya, V43, P1496
[3] BRODY TM, 1952, J BIOL CHEM, V195, P685
[4] COLLINS G G S, 1970, Nature (London), V225, P817, DOI 10.1038/225817a0
[5] ISOELECTRIC FOCUSING IN ACRYLAMIDE GELS - USE OF AMPHOTERIC DYES AS INTERNAL MARKERS FOR DETERMINATION OF ISOELECTRIC POINTS
CONWAYJACOBS, A
LEWIN, LM
[J]. ANALYTICAL BIOCHEMISTRY, 1971, 43 (02) : 394 - +
[6] PARTIAL-PURIFICATION OF MONOAMINE-OXIDASE FROM RAT-LIVER BY CHROMATOGRAPHY ON DIAZO-COUPLED TYRAMINE-SEPHAROSE
DENNICK, RG
MAYER, RJ
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1976, 4 (02) : 344 - 346
[7] EKSTEDT B, 1976, ARCH INT PHARMACOD T, V222, P157
[8] TRITON X-100-4M UREA AS AN EXTRACTION MEDIUM FOR MEMBRANE PROTEINS .1. PURIFICATION OF CHLOROPLAST CYTOCHROME-B 559
GAREWAL, HS
WASSERMAN, AR
[J]. BIOCHEMISTRY, 1974, 13 (20) : 4063 - 4071
[9] THE HISTOCHEMICAL DEMONSTRATION OF MONOAMINE OXIDASE ACTIVITY BY TETRAZOLIUM SALTS
GLENNER, GG
BURTNER, HJ
BROWN, GW
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1957, 5 (06) : 591 - 600
[10] PARTIAL SEPARATION OF RAT LIVER MITOCHONDRIAL AMINE OXIDASES
GORKIN, VZ
[J]. NATURE, 1963, 200 (490) : 77 - +

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