INSTANTANEOUS MEASUREMENT OF GLOMERULAR-FILTRATION RATE IN THE ISOLATED PERFUSED RAT-KIDNEY

被引：18

作者：

BRINK, HM

SLEGERS, JFG

机构：

[1] Department of Physiology, University of Nijmegen, Nijmegen

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1979年 / 383卷 / 01期

关键词：

Colorimeter; Flow meter; GFR-monitoring; Vitamin B[!sub]12[!/sub] clearance;

D O I：

10.1007/BF00584477

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

In perfusion studies on the isolated cellfree perfused rat kidney, the glomerular filtration rate (GFR) can be controlled if the GFR can be measured instantaneously. This paper reports on a GFR meter which opens this possibility. Vitamin B12 is used as a marker for the GFR. A bubble flow meter determines urine flow rates ranging from 10 up to 200 μl · min-1. A fiberoptic colorimeter measures vitamin B12 concentrations in the urine up to 400 mg · l-1. The flow meter and the colorimeter are described in detail. The reliability of vitamin B12 as a marker for the GFR is demonstrated and, moreover, the identity of GFR values obtained with the GFR meter and those from B12 spectrophotometry is demonstrated. © 1979 Springer-Verlag.

引用

页码：71 / 73

页数：3

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