DETECTION AND LIFETIME MEASUREMENT OF SINGLE MOLECULES IN FLOWING SAMPLE STREAMS BY LASER-INDUCED FLUORESCENCE

被引：99

作者：

WILKERSON, CW ^{[1
]}

GOODWIN, PM ^{[1
]}

AMBROSE, WP ^{[1
]}

MARTIN, JC ^{[1
]}

KELLER, RA ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB,DIV LIFE SCI,LOS ALAMOS,NM 87545

来源：

APPLIED PHYSICS LETTERS | 1993年 / 62卷 / 17期

关键词：

D O I：

10.1063/1.109495

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The detection and measurement of fluorescence lifetimes of single Rhodamine-110 molecules in a flowing, aqueous sample stream is described. Time-correlated single-photon counting, used in combination with mode-locked picosecond pulsed excitation, allows the detection of single fluorescent molecules in the presence of significant solvent Raman and Rayleigh backgrounds. The fluorescence lifetime of a detected molecule is estimated from the record of arrival times (relative to the excitation pulse) of photons detected during the molecule's passage through the approximately 1 pL excitation volume.

引用

页码：2030 / 2032

页数：3

共 5 条

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