HPLC AND FAB MASS-SPECTROMETRY ANALYSIS OF FUMONISINS B1 AND B2 PRODUCED BY FUSARIUM-MONILIFORME ON FOOD SUBSTRATES

被引：39

作者：

HOLCOMB, M ^{[1
]}

SUTHERLAND, JB ^{[1
]}

CHIARELLI, MP ^{[1
]}

KORFMACHER, WA ^{[1
]}

THOMPSON, HC ^{[1
]}

LAY, JO ^{[1
]}

HANKINS, LJ ^{[1
]}

CERNIGLIA, CE ^{[1
]}

机构：

[1] UNIV ARKANSAS,LITTLE ROCK,AR 72204

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1993年 / 41卷 / 03期

关键词：

D O I：

10.1021/jf00027a004

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Flasks containing moistened, autoclaved corn, unmilled rice, peanuts, soybeans, and laboratory rodent feed were inoculated with Fusarium moniliforme NRRL 13616 and incubated in the dark at 25-degrees-C. After 24 days, the cultures were extracted with acetonitrile and water. High-performance liquid chromatography (HPLC) of the extracts, after they had been derivatized with fluorescamine, showed high concentrations of fumonisins B1 and B2 (10 242 and 3068 mug/g, respectively) in the corn cultures and moderately high concentrations (206 and 100 mug/g, respectively) in the unmilled rice cultures. HPLC also detected fumonisins B1 and B2 (34 and 50 mug/g, respectively) in the rodent feed cultures. Only trace levels (5 mug/g or less) of fumonisin B1 were detected in the peanut and soybean cultures. Fast atom bombardment mass spectrometry (FAB MS) confirmed the presence of fumonisins B1 and B2 in the corn, unmilled rice, and rodent feed cultures.

引用

页码：357 / 360

页数：4

共 18 条

[1] BIOHERBICIDAL POTENTIAL OF FUSARIUM-MONILIFORME AND ITS PHYTOTOXIN, FUMONISIN [J].