REQUIREMENT FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN EPIDERMAL GROWTH-FACTOR SIGNALING

被引：156

作者：

KAUFFMANNZEH, A

THOMAS, GMH

BALL, A

PROSSER, S

CUNNINGHAM, E

COCKCROFT, S

HSUAN, JJ

机构：

[1] UCL, SCH MED, LUDWIG INST CANC RES, PROT BIOCHEM GRP, LONDON W1P 8BT, ENGLAND

[2] UCL, DEPT PHYSIOL, LONDON WC1E 6JJ, ENGLAND

来源：

SCIENCE | 1995年 / 268卷 / 5214期

基金：

英国惠康基金;

关键词：

D O I：

10.1126/science.7761838

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Stimulation of phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis is a widespread mechanism for receptor-mediated signaling in eukaryotes. Cytosolic phosphatidylinositol transfer protein (PITP) is necessary for guanosine triphosphate (GTP)-dependent hydrolysis of PIP2 by phospholipase C-beta (PLC-beta), but the role of PITP is unclear. Stimulation of phospholipase C-gamma (PLC-gamma) in A431 human epidermoid carcinoma cells treated with epidermal growth factor (EGF) required PITP. Stimulation of PI-4 kinase in cells treated with EGF also required PITP. Coprecipitation studies revealed an EGF-dependent association of PITP with the EGF receptor, with PI-4 kinase, and with PLC-gamma.

引用

页码：1188 / 1190

页数：3

共 40 条

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