ANALYSIS OF ERRORS IN FINISHED DNA-SEQUENCES - THE SURFACTIN OPERON OF BACILLUS-SUBTILIS AS AN EXAMPLE

被引：8

作者：

FABRET, C ^{[1
]}

QUENTIN, Y ^{[1
]}

GUISEPPI, A ^{[1
]}

BUSUTTIL, J ^{[1
]}

HAIECH, J ^{[1
]}

DENIZOT, F ^{[1
]}

机构：

[1] CNRS,CHIM BACTERIENNE LAB,F-13277 MARSEILLE 9,FRANCE

来源：

MICROBIOLOGY-UK | 1995年 / 141卷

关键词：

BACILLUS SUBTILIS; GENOME SEQUENCING; ACCURACY; ERROR; SURFACTIN;

D O I：

10.1099/13500872-141-2-345

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Increased productivity in DNA sequencing would not be valid without a straightforward detection and estimation of errors in finished sequences, The sequence of the surfactin operon from Bacillus subtilis was obtained by two different groups and by chance we were also working on the same chromosome region. Taking advantage of this situation we report in this paper, the number and nature of errors found in the overlapping part of the DNA sequences obtained by the three laboratories. The coincidence of some of the errors with compression in sequence ladders and with secondary DNA structures as well as the detection of frameshift errors using computer programs, are demonstrated. Finally we discuss the definition of a new sequencing strategy that might minimize both the error rate and the cost of sequencing.

引用

页码：345 / 350

页数：6