DIFFERENTIAL-EFFECTS OF AN ACYL-COENZYME A-CHOLESTEROL ACYLTRANSFERASE INHIBITOR ON HDL-INDUCED CHOLESTEROL EFFLUX FROM RAT MACROPHAGE FOAM CELLS

被引：21

作者：

HAKAMATA, H ^{[1
]}

MIYAZAKI, A ^{[1
]}

SAKAI, M ^{[1
]}

SAKAMOTO, YI ^{[1
]}

MATSUDA, H ^{[1
]}

KIHARA, K ^{[1
]}

HORIUCHI, S ^{[1
]}

机构：

[1] KUMAMOTO UNIV,SCH MED,DEPT BIOCHEM,KUMAMOTO 860,JAPAN

来源：

FEBS LETTERS | 1995年 / 363卷 / 1-2期

关键词：

MACROPHAGE FOAM CELL; CHOLESTERYL ESTER CYCLE; ACAT INHIBITOR; HIGH DENSITY LIPOPROTEIN; CHOLESTEROL EFFLUX; INTRACELLULAR CHOLESTEROL TRANSPORT;

D O I：

10.1016/0014-5793(95)00273-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When rat macrophages were converted to foam cells with acetylated low density lipoprotein (acetyl-LDL) and then reacted with high density lipoprotein (HDL) and an inhibitor of acyl-coenzyme A:cholesterol acyltransferase (58-035) (sequential incubation system), 58-035 did not enhance HDL-induced cholesterol efflux. In contrast, when macrophages were exposed to acetyl-LDL in the presence of both HDL and 58-035 (simultaneous incubation system), HDL-induced cholesterol efflux was enhanced 1.6-fold by 58-035. Cholesterol efflux with HDL alone was 2-fold greater in simultaneous incubation than in sequential incubation. These results suggest the presence of an efficient cholesterol efflux pathway in simultaneous incubation which is not available in sequential incubation. This pathway, which we refer to as the neutral cholesterol ester hydrolase-independent pathway, is characterized by the efflux of lysosome-derived cholesterol without re-esterification.

引用

页码：29 / 32

页数：4

共 22 条

[1] INFLUENCE OF HIGH-DENSITY LIPOPROTEIN ON ESTERIFIED CHOLESTEROL STORES IN MACROPHAGES AND HEPATOMA-CELLS [J].

BERNARD, DW ;

RODRIGUEZ, A ;

ROTHBLAT, GH ;

GLICK, JM .

ARTERIOSCLEROSIS, 1990, 10 (01) :135-144

[2] COMPARISON OF CI-976, AN ACAT INHIBITOR, AND SELECTED LIPID-LOWERING AGENTS FOR ANTIATHEROSCLEROTIC ACTIVITY IN ILIAC FEMORAL AND THORACIC AORTIC LESIONS - A BIOCHEMICAL, MORPHOLOGICAL, AND MORPHOMETRIC EVALUATION [J].

BOCAN, TMA ;

MUELLER, SB ;

UHLENDORF, PD ;

NEWTON, RS ;

KRAUSE, BR .

ARTERIOSCLEROSIS AND THROMBOSIS, 1991, 11 (06) :1830-1843

[3]

BRASAEMLE DL, 1990, J LIPID RES, V31, P103

[4]

BROWN MS, 1980, J BIOL CHEM, V255, P9344

[5]

DORY L, 1989, J LIPID RES, V30, P809

[6] SPECIES-DIFFERENCE IN CHOLESTERYL ESTER CYCLE AND HDL-INDUCED CHOLESTEROL EFFLUX FROM MACROPHAGE FOAM CELLS [J].

HAKAMATA, H ;

MIYAZAKI, A ;

SAKAI, M ;

SUGINOHARA, Y ;

SAKAMOTO, YI ;

HORIUCHI, S .

ARTERIOSCLEROSIS AND THROMBOSIS, 1994, 14 (11) :1860-1865

[7]

HEIDER JG, 1978, J LIPID RES, V19, P514

[8] MODULATING EFFECTS OF CANINE HIGH-DENSITY LIPOPROTEINS ON CHOLESTERYL ESTER SYNTHESIS INDUCED BY BETA VERY LOW-DENSITY LIPOPROTEINS IN MACROPHAGES - POSSIBLE INVITRO CORRELATES WITH ATHEROSCLEROSIS [J].

INNERARITY, TL ;

PITAS, RE ;

MAHLEY, RW .

ARTERIOSCLEROSIS, 1982, 2 (02) :114-124

[9] THE INTRACELLULAR STORAGE AND TURNOVER OF APOLIPOPROTEIN-B OF OXIDIZED LDL IN MACROPHAGES [J].

JESSUP, W ;

MANDER, EL ;

DEAN, RT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1126 (02) :167-177

[10]

JOHNSON WJ, 1990, J BIOL CHEM, V265, P5546

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