ISOLATION OF FETAL TROPHOBLAST CELLS FROM PERIPHERAL-BLOOD OF PREGNANT-WOMEN

被引：154

作者：

MUELLER, UW ^{[1
]}

HAWES, CS ^{[1
]}

WRIGHT, AE ^{[1
]}

PETROPOULOS, A ^{[1
]}

DEBONI, E ^{[1
]}

FIRGAIRA, FA ^{[1
]}

MORLEY, AA ^{[1
]}

TURNER, DR ^{[1
]}

JONES, WR ^{[1
]}

机构：

[1] FLINDERS UNIV,MED CTR,DEPT HAEMATOL,BEDFORD PK,SA 5042,AUSTRALIA

来源：

LANCET | 1990年 / 336卷 / 8709期

关键词：

D O I：

10.1016/0140-6736(90)91731-O

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Fetal trophoblast cells were isolated from maternal peripheral blood by means of murine monoclonal antibodies of high specificity and affinity for human syncytiotrophoblast and nonvillous cytotrophoblast cells. The cells were isolated in sufficient numbers to allow polymerase chain reaction (PCR) amplification of the Y-chromosome-specific DNA sequence from the peripheral blood of thirteen pregnant women. The fetal sex predicted by PCR analysis of the isolated trophoblast cells accorded with that ascertained by karyotyping of chorionic villus samples in eleven of twelve women studied in early pregnancy and with the sex of the baby on delivery in one woman studied at 34 weeks' gestation. Isolation of these fetal cells could allow non-invasive diagnosis of a wide range of inherited disorders. © 1990.

引用

页码：197 / 200

页数：4

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