STRATEGIES FOR IMPROVING PLASMID STABILITY IN GENETICALLY MODIFIED BACTERIA IN BIOREACTORS

被引：76

作者：

KUMAR, PKR ^{[1
]}

MASCHKE, HE ^{[1
]}

FRIEHS, K ^{[1
]}

SCHUGERL, K ^{[1
]}

机构：

[1] UNIV BIELEFELD, AG FERMENTATIONSTECH, FAK TECH, W-4800 BIELEFELD 1, GERMANY

来源：

TRENDS IN BIOTECHNOLOGY | 1991年 / 9卷 / 08期

关键词：

D O I：

10.1016/0167-7799(91)90090-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Exploitation of recombinant organisms for the large-scale, commercial production of foreign proteins is often hampered by the problem of plasmid instability. A wide range of strategies have been reported for improving the stability of recombinant organisms. A combination of manipulating both the genetic design of recombinants and the conditions of culturing the organisms may be used to achieve stable host-vector associations during culture of recombinant organisms in bioreactors.

引用

页码：279 / 284

页数：6

共 53 条

[1] EFFECTS OF TEMPERATURE ON PLASMID STABILITY AND PENICILLINASE PRODUCTIVITY IN A TRANSFORMANT OF BACILLUS-STEAROTHERMOPHILUS [J].

AIBA, S ;

KOIZUMI, J .

BIOTECHNOLOGY AND BIOENGINEERING, 1984, 26 (09) :1026-1031

[2] PLASMID PARTITION [J].

AUSTIN, SJ .

PLASMID, 1988, 20 (01) :1-9

[3]

CAULCOTT CA, 1987, J GEN MICROBIOL, V133, P1881

[4] THE EFFECT OF OXYGEN LIMITATION ON STABILITY OF A RECOMBINANT PLASMID IN SACCHAROMYCES-CEREVISIAE [J].

CAUNT, P ;

IMPOOLSUP, A ;

GREENFIELD, PF .

BIOTECHNOLOGY LETTERS, 1989, 11 (01) :5-10

[5] STABILITY OF PBR322-DERIVED PLASMIDS [J].

CHIANG, CS ;

BREMER, H .

PLASMID, 1988, 20 (03) :207-220

[6] IDENTIFICATION AND CLASSIFICATION OF BACTERIAL PLASMIDS [J].

COUTURIER, M ;

BEX, F ;

BERGQUIST, PL ;

MAAS, WK .

MICROBIOLOGICAL REVIEWS, 1988, 52 (03) :375-395

[7]

DIERICHSEN B, 1986, MOL GENETICS BIOTECH, P35

[8] REPLICATION OF R-FACTOR R1 IN ESCHERICHIA-COLI K-12 AT DIFFERENT GROWTH-RATES [J].

ENGBERG, B ;

NORDSTROM, K .

JOURNAL OF BACTERIOLOGY, 1975, 123 (01) :179-186

[9] STABILITY OF RECOMBINANT PLASMIDS IN INDUSTRIAL MICROORGANISMS [J].

ENSLEY, BD .

CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, 1986, 4 (03) :263-277

[10] OPTIMIZING THE PRODUCTION OF RECOMBINANT PROTEINS IN MICROORGANISMS [J].

GEORGIOU, G .

AICHE JOURNAL, 1988, 34 (08) :1233-1248

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