PRODUCTION AND CHARACTERIZATION OF 2 NEW MOUSE MONOCLONAL-ANTIBODIES REACTIVE WITH DENATURED MOUSE CLASS-II BETA-CHAINS

We report on the production and characterization of two murine peptide specific anti-major histocompatibility complex class II chain specific monoclonal antibodies. Two new mouse monoclonal antibodies reactive with two synthetic peptides corresponding to Al(b) amino acids (146-157) and Ab(s) amino acids(146-157) were produced. KL295 is the mouse anti-Ab(b) monoclonal antibody, which reacts with denatured beta-chains of H-2b, H-2d, H-2P, and H-2q, but fail to react with spleen cell lysates from H-2f, H-2j, H-2k and H-2s mice The mouse anti Ab(s) monoclonal antibody, KL304 in contrast reacts with the denatured Class II beta-chains of H-2f, H-2j, H-2k and H-2s mice, but fails to bind H-2b, H-2d, H-2P or H-2q beta-chain, suggesting residues 153-155 of this molecule to be critical for the epitope. Both KL295 and KL304 bind BIO.WB (H-2J) which suggests a unique epitope in this strain of mice. Neither KL295 or KL304 react with native mouse class II cell surface molecules.