IMPROVED DETECTION OF FETAL CELLS FROM MATERNAL BLOOD WITH POLYMERASE CHAIN-REACTION

被引:13
作者
ADKISON, LR [1 ]
ANDREWS, RH [1 ]
VOWELL, NL [1 ]
KOONTZ, WL [1 ]
机构
[1] MED CTR CENT GEORGIA,DEPT OBSTET & GYNECOL,MACON,GA
关键词
FETAL SEX DETERMINATION; PERIPHERAL MATERNAL BLOOD; POLYMERASE CHAIN REACTION;
D O I
10.1016/S0002-9378(94)70312-4
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: The objective was to test the reliability of new deoxyribonucleic acid primers that have previously been used very efficiently by this laboratory with amniolysate samples to amplify a 248 bp Y-specific, repeated sequence from maternal blood during pregnancy. STUDY DESIGN: Blood samples were obtained from 50 women during weeks 11 and 16 of pregnancy and were analyzed for the presence of the Y chromosome-specific sequences. RESULTS: Y-specific fragments were identified in 19 of 24 (79.2%) women after one complete amplification. A second amplification of these samples negative for Y-specific fragments revealed three additional samples positive for the Y chromosome-specific fragment. Only two male fetuses remained unidentified. Overall, 91.7% male fetuses and 96% of all fetuses (48/50) in these women were correctly identified. CONCLUSIONS: The primers described in this study provide an additional or alternative tool for the determination, by means of the polymerase chain reaction, of Y chromosome-bearing cells in maternal circulation.
引用
收藏
页码:952 / 955
页数:4
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