GENETIC-TRANSFORMATION IN HELICOBACTER-PYLORI

被引：43

作者：

TSUDA, M ^{[1
]}

KARITA, M ^{[1
]}

NAKAZAWA, T ^{[1
]}

机构：

[1] YAMAGUCHI UNIV,SCH MED,DEPT INTERNAL MED 1,UBE,YAMAGUCHI 755,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1993年 / 37卷 / 01期

关键词：

HELICOBACTER-PYLORI; TRANSFORMATION; ELECTROPORATION;

D O I：

10.1111/j.1348-0421.1993.tb03184.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Genetic transformation in Helicobacter pylori was investigated by using its chromosomal and plasmid DNAs. Six out of the eight strains exhibited the natural competence for incorporation of H. pylori chromosomal DNA, and all the strains incorporated the donor DNA efficiently by washing and concentrating the cells with a glycerol solution. The much higher frequency of transformation was obtained in each strain by means of electroporation. Electroporation experiments were also conducted by use of the recombinant DNAs consisting of the H. pylori and Escherichia coli plasmids as the donors, and the occurrence of the homologous recombination was demonstrated between the incoming H. pylori plasmid-derived region and the corresponding region of the originally residing plasmid in H. pylori.

引用

页码：85 / 89

页数：5

共 17 条

[1] HELICOBACTER-PYLORI AND THE PATHOGENESIS OF GASTRODUODENAL INFLAMMATION [J].