IMPROVED RAPID METHOD FOR PRODUCTION AND PURIFICATION OF CLOSTRIDIUM-PERFRINGENS TYPE-A ENTEROTOXIN

被引：7

作者：

HEREDIA, NL

GARCIAALVARADO, JS

LABBE, RG

机构：

[1] UNIV MASSACHUSETTS, DEPT FOOD SCI, AMHERST, MA 01003 USA

[2] UNIV AUTONOMA NUEVO LEON, FAC CIENCIAS BIOL, DEPT MICROBIOL & INMUNOL, SAN NICOLAS, NUEVO LEON, MEXICO

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1994年 / 20卷 / 02期

关键词：

CLOSTRIDIUM PERFRINGENS; ENTEROTOXIN; SPORULATION;

D O I：

10.1016/0167-7012(94)90011-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Clostridium perfringens strain FD-1041 was grown in Duncan-Strong medium containing raffinose for 5 h at 43 degrees C. Enterotoxin in the cell extract was purified 5.5-fold by a modified ammonium sulfate-gel filtration method using Sephacryl S-200. The enterotoxin could be purified to homogeneity with 50% recovery in less than 48 h.

引用

页码：87 / 91

页数：5

共 22 条

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