REVERSE TRANSCRIPTION PCR DETECTION OF LACROSSE VIRUS IN MOSQUITOS AND COMPARISON WITH ENZYME-IMMUNOASSAY AND VIRUS ISOLATION

被引:19
作者
WASIELOSKI, LP [1 ]
RAYMSKELLER, A [1 ]
CURTIS, LA [1 ]
BLAIR, CD [1 ]
BEATY, BJ [1 ]
机构
[1] COLORADO STATE UNIV,DEPT MICROBIOL,ARTHROPOD BORNE & INFECT DIS LAB,FT COLLINS,CO 80523
关键词
D O I
10.1128/JCM.32.9.2076-2080.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A reverse transcription-PCR (RT-PCR) assay was developed and compared with enzyme immunoassay (EIA) and virus isolation for detecting LaCrosse virus (LAC) in mosquito pools. All three techniques were able to detect a single LAC-infected mosquito in a pool of 99 negative mosquitoes. Virus isolation was the most sensitive of the three techniques; it was possible to isolate virus immediately following intrathoracic inoculation of mosquitoes. RT-PCR was second in sensitivity; LAC RNA was detected 1 day postinfection. EIA detected LAC antigen 2 days postinfection. Additionally, RT-PCR and EIA were able to detect LAC RNA and protein, respectively, from mosquito samples which were subjected to seven freeze-thaw cycles, and RT-PCR was able to detect LAC RNA from mosquito samples which remained at room temperature for up to 7 days.
引用
收藏
页码:2076 / 2080
页数:5
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