PURIFICATION AND SOME PROPERTIES OF 6 CHITINASES FROM AEROMONAS SP NO 10S-24

被引：49

作者：

UEDA, M

FUJIWARA, A

KAWAGUCHI, T

ARAI, M

机构：

[1] Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 11期

关键词：

D O I：

10.1271/bbb.59.2162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Six chitinases were purified from a culture supernatant of Aeromonas sp. no. 10S-24 bq ammonium sulfate precipitation, DEAE-Sephadex A-50, Butyl-Toyopearl 650M, and chromatofocusing, These enzymes were most active at pH 3.5-4.5 and the optimum temperature were 50 degrees C. The molecular weights of the enzymes were 89,000 to 120,000 from SDS-polyacrylamide gel electrophoresis. N-Terminal amino acid sequences of the enzymes were similar to that of chitinase I.

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页码：2162 / 2164

页数：3

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