NUCLEOTIDE INHIBITION OF ADENYL CYCLASE ACTIVITY IN FAT CELL MEMBRANES

被引：77

作者：

CRYER, PE

JARETT, L

KIPNIS, DM

机构：

[1] Washington University School of Medicine, Departments of Medicine and Pathology, St. Louis, MO

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 177卷 / 03期

关键词：

D O I：

10.1016/0304-4165(69)90323-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A reproducible assay for adenyl cyclase activity in an isolated fat cell membrane preparation is described. Enzyme activity was stimulated by epinephrine and sodium fluoride, but not affected by insulin. GTP, GDP, GMP, CTP and UTP inhibited the adenyl cyclase activity of the fat cell membrane, but guanosine and cyclic GMP had no effect. In contrast to the inhibitory effect on the fat cell membrane enzyme, GTP did not affect either adrenal or kidney adenyl cyclase activity. © 1969.

引用

页码：586 / &

共 15 条

[1] BOYER PD, 1962, ENZYMES, V6, P96
[2] RENAL ADENYL CYCLASE - ANATOMICALLY SEPARATE SITES FOR PARATHYROID HORMONE AND VASOPRESSIN
CHASE, LR
AURBACH, GD
[J]. SCIENCE, 1968, 159 (3814) : 545 - &
[3] COOK JL, 1966, J BIOL CHEM, V241, P158
[4] SPECIFICITY OF PYRUVATE KINASE
DAVIDSON, EA
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1959, 33 (01) : 238 - 240
[5] IN VITRO REGULATION OF LIPOLYSIS OF ADIPOSE TISSUE
DAVIES, JI
[J]. NATURE, 1968, 218 (5139) : 349 - &
[6] ADENYL CYCLASE OF BREVIBACTERIUM LIQUEFACIENS
HIRATA, M
HAYAISHI, O
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 149 (01) : 1 - &
[7] LOWRY OH, 1951, J BIOL CHEM, V193, P265
[8] ROLE OF ADENOSINE 3',5'-PHOSPHATE IN ACTION OF ANTIDIURETIC HORMONE
ORLOFF, J
HANDLER, J
[J]. AMERICAN JOURNAL OF MEDICINE, 1967, 42 (05) : 757 - &
[9] ADENYL CYCLASE AS AN ADRENERGIC RECEPTOR
ROBISON, GA
BUTCHER, RW
SUTHERLA.EW
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1967, 139 (A3) : 703 - &
[10] RODBELL M, 1967, J BIOL CHEM, V242, P5744

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