NEW MICRO-ASSAY FOR HYALURONIDASE ACTIVITY

被引：9

作者：

CHEN, SS

HSU, DS

HOFFMAN, P

机构：

[1] SUNY STONY BROOK, SCH MED, DEPT OBSTET & GYNECOL, STONY BROOK, NY 11794 USA

[2] SUNY STONY BROOK, SCH MED, DEPT ORTHOPED SURG, STONY BROOK, NY 11794 USA

来源：

CLINICA CHIMICA ACTA | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0009-8981(79)90369-3

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A new micro-method for the assay of hyaluronidase activity is described. The method utilizes chondroitin sulfate as a substrate. The degraded products of chondroitin sulfate by hyaluronidase are determined by modified disc gel electrophoresis. The products are first concentrated into a single band in acrylamide gel, and then the band is stained with cetylpyridinium chloride. The absorbance of the band is proportional to the amount of the degraded products. The hyaluronidase activity is therefore linearly related to the absorbance. This procedure represents a sensitive method for the assay of hyaluronidase activity in the serum and urine. © 1979.

引用

页码：277 / 284

页数：8

共 19 条

[1]

ARONSON NN, 1967, J BIOL CHEM, V242, P437

[2] URINARY HYALURONIDASE [J].