RAPID COLUMN CHROMATOGRAPHIC METHOD FOR THE ISOLATION OF CATECHOL-TYPE SIDEROPHORES

被引：6

作者：

ROBINSON, AV

机构：

[1] Biology Department, Battelle, Pacific Northwest Laboratory, Richland

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90740-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A column chromatographic method using a polyamide support has been developed which allows rapid isolation of catechol-type siderophores directly from culture supernatants. The method is superior to presently used methods with regard to speed, convenience, and lack of degradation products. Siderophores from Micrococous denitrificans (ATCC 19367). Klebsiella acrogenes (ATCC 13882), and Bacillus subtilis (ATCC 6051) have been purified and the method has been tested with two synthetic catechol-type chelators, N1,N2-bis(2,3-dihydroxybenzoyl)ethylenediamine and N1,N4-bis(2,3-dihydroxybenzoyl)putrescine. © 1979.

引用

页码：364 / 370

页数：7

共 13 条

[1]

BROT N, 1966, BIOCHEM BIOPH RES CO, V25, P455

[2]

CHABEREK S, 1959, ORGANIC SEQUESTERING, P572

[3] ISOLATION AND IDENTIFICATION OF 2,3-DIHYDROXYBENZOIC ACID AND 2-N,6-N-DI-(2,3-DIHYDROXYBENZOYL)-L-LYSINE FORMED BY IRON-DEFICIENT AZOTOBACTER VINELANDII [J].