CONSTRUCTION OF A 2.6-MB CONTIG IN YEAST ARTIFICIAL CHROMOSOMES SPANNING THE HUMAN DYSTROPHIN GENE USING AN STS-BASED APPROACH

被引：93

作者：

COFFEY, AJ

ROBERTS, RG

GREEN, ED

COLE, CG

BUTLER, R

ANAND, R

GIANNELLI, F

BENTLEY, DR

机构：

[1] WASHINGTON UNIV, SCH MED, DEPT GENET, ST LOUIS, MO 63110 USA

[2] ICI PHARMACEUT PLC, DEPT BIOTECHNOL, MACCLESFIELD, CHESHIRE, ENGLAND

来源：

GENOMICS | 1992年 / 12卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0888-7543(92)90437-W

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A sequence tagged site (STS)-based approach has been used to construct a 2.6-Mb contig in yeast artificial chromosomes (YACs) spanning the human dystrophin gene. Twenty-seven STSs were used to identify and overlap 34 YAC clones. A DNA fingerprint of each clone produced by direct Alu-PCR amplification of YAC colonies and the isolation of YAC insert ends by vectorette PCR were used to detect overlaps in intron 1 (280 kb) where no DNA sequence data were available, thereby achieving closure of the map. This study has evaluated methods for mapping large regions of the X chromosome and provides a valuable resource of the dystrophin gene in cloned form for detailed analysis of gene structure and function in the future. © 1992.

引用

页码：474 / 484

页数：11

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[1] A CONVENIENT MULTIPLEX PCR SYSTEM FOR THE DETECTION OF DYSTROPHIN GENE DELETIONS - A COMPARATIVE-ANALYSIS WITH CDNA HYBRIDIZATION SHOWS MISTYPINGS BY BOTH METHODS [J].