POLYMERASE CHAIN REACTION-AMPLIFIED NONRADIOACTIVE PROBES FOR IDENTIFICATION OF FUSOBACTERIUM-NUCLEATUM

被引：16

作者：

BOLSTAD, AI ^{[1
]}

JENSEN, HB ^{[1
]}

机构：

[1] UNIV BERGEN,SCH DENT,ORAL MICROBIOL LAB,N-5014 BERGEN,NORWAY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 03期

关键词：

D O I：

10.1128/JCM.31.3.528-532.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A polymerase chain reaction probe with 100% sequence identity to 120 deoxyribonucleotides of Fusobacterium nucleatum Fev1, coding for a part of the 40-kDa major outer membrane protein, was labeled with the steroid hapten digoxigenin. The probe was compared with various degenerate oligonucleotide probes and found to tolerate much more stringent washing conditions. It was therefore superior in distinguishing, by means of Southern blots and slot blots, F. nucleatum from other oral gram-negative bacteria in the periodontal pocket and from other fusobacterial species and in distinguishing among different strains of F. nucleatum. F. periodonticum was found to be more similar to F. nucleatum than the other fusobacterial species tested.

引用

页码：528 / 532

页数：5

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