CLONING AND CHARACTERIZATION OF CDNAS FOR 70-KDA HEAT-SHOCK PROTEINS (HSP70) FROM 2 FISH SPECIES OF THE GENUS ORYZIAS

被引：37

作者：

ARAI, A ^{[1
]}

NARUSE, K ^{[1
]}

MITANI, H ^{[1
]}

SHIMA, A ^{[1
]}

机构：

[1] UNIV TOKYO,SCH SCI,DEPT SCI BIOL,RADIAT BIOL LAB,BUNKYO KU,TOKYO 113,JAPAN

来源：

JAPANESE JOURNAL OF GENETICS | 1995年 / 70卷 / 03期

关键词：

D O I：

10.1266/jjg.70.423

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

cDNA corresponding to two hsp70-related genes (OLHSC70 and CEHSC70) were isolated from two lines of cultured fish cells derived from the genus Oryzias. OLHSC70 was 2,261 bp in length and encoded a protein of 686 amino acids with a predicted molecular mass of 76,120 daltons. CEHSC70 was 2,114 bp in length and it lacked the 5' region found in OLHSC70. Two-dimensional electrophoresis revealed that Oryzias latipes has at least three heat-inducible proteins with molecular masses of about 70,000 daltons. One of these proteins (Hsp70.1) was barely expressed under normal conditions but its high-level expression was induced by hyperthermia. The other two proteins (Hsc70.1, and Hsc70.2) were constitutively expressed under normal conditions and only slightly enhanced levels were induced by hyperthermia. Transfection with the cloned sequence, RNA dot-blot analysis and the two-dimensional electrophoresis of proteins showed that OLHSC70 encoded Hsc70.1.

引用

页码：423 / 433

页数：11

共 28 条

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