ISOLATION AND PARTIAL CHARACTERIZATION OF A CHYMOTRYPSIN-LIKE ENDOPROTEASE FROM COCKROACH INTESTINAL SYSTEM

被引：22

作者：

BAUMANN, E ^{[1
]}

机构：

[1] SACHSISCHE AKAD WISSENSCH LEIPZIG, O-6900 JENA, GERMANY

来源：

INSECT BIOCHEMISTRY | 1990年 / 20卷 / 08期

关键词：

COCKROACH; CHYMOTRYPSIN; INHIBITOR SPECIFICITY; INSULIN CLEAVAGE SITE; PROTEASE;

D O I：

10.1016/0020-1790(90)90093-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The caeca were demonstrated to contain the highest proteolytic activity of the cockroach intestinal system. Nearly twice the enzymatic activity of males was found in females. A protease with chymotrypsin-like specificities and a molecular mass of around 25 kDa was purified nearly to homogeneity by gel filtration and ion exchange chromatography on DEAE- and CM-cellulose. The N-terminal amino acid has been identified as isoleucin by using dansyl chloride. The enzyme seems to cleave the insulin B chain preferentially on the carboxyl side of aromatic and hydrophobic amino acids.

引用

页码：761 / 768

页数：8

共 32 条

[1]

AKIMENKO Z A, 1988, Biokhimiya, V53, P1111

[2]

Allen G., 1981, SEQUENCING PROTEINS

[3]

CHARACTERIZATION OF PROTEOLYTIC-ENZYMES OF THE MIDGUT AND EXCRETA OF THE BITING FLY STOMOXYS-CALCITRANS [J].

BOROVSKY, D .

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 1985, 2 (02) :145-159

[4]

QUANTITATIVE-DETERMINATION OF TRYPSIN-LIKE AND CHYMOTRYPSIN-LIKE ENZYMES IN INSECTS [J].

BOROVSKY, D ;

SCHLEIN, Y .

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 1988, 8 (04) :249-260

[5]

Dow AT, 1987, ADV INSECT PHYSIOL, V19, P187, DOI [10.1016/S0065-2806(08)60102-2, DOI 10.1016/S0065-2806(08)60102-2]

[6]

PURIFICATION AND PROPERTIES OF A CHYMOTRYPSIN INHIBITOR FROM THE SILKWORM HEMOLYMPH [J].

EGUCHI, M ;

SHOMOTO, K .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1985, 81 (02) :301-307

[7]

SULFONYL FLUORIDES AS INHIBITORS OF ESTERASES .2. FORMATION + REACTIONS OF PHENYLMETHANESULFONYL ALPHA-CHYMOTRYPSIN [J].

GOLD, AM ;

FAHRNEY, D .

BIOCHEMISTRY, 1964, 3 (06) :783-&

[8]

TRYPSIN AND CHYMOTRYPSIN FROM BEETLE PTEROSTICHUS MELANARIUS [J].

GOODING, RH ;

HUANG, CT .

JOURNAL OF INSECT PHYSIOLOGY, 1969, 15 (02) :325-+

[9]

GRAF R, 1986, CELL TISSUE RES, V245, P19, DOI 10.1007/BF00218082

[10]

ISOLATION OF TRYPSIN ISOZYMES FROM THE MOSQUITO AEDES-AEGYPTI (L) [J].

GRAF, R ;

BRIEGEL, H .

INSECT BIOCHEMISTRY, 1985, 15 (05) :611-618

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