QUANTITATIVE IMMUNOPEROXIDASE PROCEDURE EMPLOYING ENERGY DISPERSIVE-X-RAY ANALYSIS

被引：13

作者：

SIEGESMUND, KA ^{[1
]}

YORDE, DE ^{[1
]}

DRAGEN, R ^{[1
]}

机构：

[1] MED COLL WISCONSIN,DEPT PATHOL,MILWAUKEE,WI 53226

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1979年 / 27卷 / 09期

关键词：

D O I：

10.1177/27.9.383829

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A method is described for substituting gold for osmium as a marker in the unlabeled antibody technique. The gold marker can be detected in the light or electron microscope. The gold-labeled reaction product can be detected in lower concentrations than osmium and can be used as the basis for quantitating antigen concentrations in cells and tissues with the scanning electron microscope and energy dispersive x-ray analysis.

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页码：1226 / 1230

页数：5

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