SIMULTANEOUS VISUALIZATION OF NEUROPEPTIDE AND ACETYLCHOLINESTERASE NERVE SUBPOPULATIONS IN THE PERIVASCULAR PLEXUS

被引：19

作者：

GULBENKIAN, S ^{[1
]}

VALENCA, A ^{[1
]}

WHARTON, J ^{[1
]}

POLAK, JM ^{[1
]}

DAVIDFERREIRA, JF ^{[1
]}

机构：

[1] ROYAL POSTGRAD MED SCH, DEPT HISTOCHEM, LONDON W12 0NN, ENGLAND

来源：

HISTOCHEMICAL JOURNAL | 1991年 / 23卷 / 11-12期

关键词：

D O I：

10.1007/BF01041183

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A simple method combining indirect immunofluorescence and histochemical techniques was developed in order to demonstrate the presence of both neuropeptide immunoreactivity and acetylcholinesterase activity in the same whole-mount preparation. It was found that the two methods can be combined without interfering with one another and may be viewed and photographed simultaneously. The guinea pig basilar artery was chosen as a model tissue. While vasoactive intestinal polypeptide immunoreactivity and acetylcholinesterase activity were found to occur in the same perivascular nerve fibres, tyrosine hydroxilase, neuropeptide tyrosine and calcitonin gene-related peptide immunoreactivity were present in distinct nerve subpopulations. It is possible using this double staining procedure, to analyse the interrelationship of putative cholinergic nerves with other components of the autonomic and sensory nervous system.

引用

页码：553 / 558

页数：6

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