ENUMERATION OF INTERLEUKIN-1 ALPHA-PRODUCING AND BETA-PRODUCING CELLS BY FLOW-CYTOMETRY

被引：8

作者：

CHIKANZA, IC ^{[1
]}

CORRIGAL, V ^{[1
]}

KINGSLEY, G ^{[1
]}

PANAYI, GS ^{[1
]}

机构：

[1] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,RHEUMATOL UNIT,ST THOMAS ST,GUYS CAMPUS,LONDON SE1 9RT,ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 154卷 / 02期

关键词：

IMMUNOFLUORESCENCE; INTERLEUKIN-1; FLOW CYTOMETRY;

D O I：

10.1016/0022-1759(92)90189-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique for intracytoplasmic immunofluorescence staining to detect and quantify human interleukin-1 alpha (IL-1alpha) and beta (IL-1beta) in CD4, CD8, and CD14 positive lymphoid cells is described. Mononuclear cells stimulated in vitro with PHA to produce IL-1, were fixed and made permeable to antibodies by sequential exposure to paraformaldehyde and the detergent n-octyl-glucoside. Cytoplasmic and surface staining of both forms of IL-1 were demonstrated by indirect fluorescence using IL-1beta and IL-1alpha specific mouse monoclonal antibodies and quantified with flow cytometry.

引用

页码：173 / 178

页数：6

共 16 条

[1] ENUMERATION OF IFN-GAMMA-PRODUCING CELLS BY FLOW-CYTOMETRY - COMPARISON WITH FLUORESCENCE MICROSCOPY [J].